To check generator settings, open D8 tools, AFTER exiting from BIS. Choose on-line monitor, click on generator. The settings are usually 50kV, 30mA. You should not have to do this every time.
The goniometer will drive to zero
NB: SCALE – the rings are approx 0.1mm apart. To put the crosshairs in geometric centre, in the Video program choose tools, options, move center.
Check the beamstop is in the correct position and will not collide with the detector.
Minimise video. Close the doors together and ensure that no alarm light on the enclosure is flashing.
In the background the BIS window show the status of the instrument – green lights should be on to show client connection.- new dark image box click on “wide scan” then “drive and scan”.< p> - new dark image box click on “wide scan” then “drive and scan”.< p>
Click on Instrument which is on the menu bar at the top
(frames should now be collecting in monitor experiment window) – whilst you are waiting enter experimental details in the SETUP – describe menu
Operation: Position; Active: yes; distance 100; 2 theta: 0 omega: 0; phi: 0 chi: 54.74
If there are any cells coloured pink, it is usually because the angle is greater than 360. This is OK
If you need to interrupt the experiment go to Instrument – choose abort
You need to have determined the unit cell to do this step (If you are unsure of the Laue symmetry – make precession photos, including level 1, from the full dataset – this is under “evaluate crystal”)
Good data has spot correlation coefficient tending to oneWhen integration has finished, it will say integration finished in the top right hand corner box
Links to the other X-Ray Crystallography web pages are also listed here for your convenience:-